Improved Cold and Drought Tolerance of Doubled Haploid Maize Plants Selected for Resistance to Prooxidant tert‐Butyl Hydroperoxide     

É. Darkó  J. Fodor  S. Dulai  H. Ambrus  A. Szenzenstein  Z. Király  B. Barnabás 《Journal of Agronomy and Crop Science》2011,197(6):454-465

To improve the abiotic stress tolerance of maize (Zea mays L.), doubled haploid (DH) plants were produced by in vitro selection of microspores exposed to tert‐butyl hydroperoxide (t‐BuOOH) as a powerful prooxidant This study investigated the tolerance of the progenies of t‐BuOOH‐selected DH lines to oxidative stress, cold and drought in controlled environment pot experiments by analyses of photosynthetic electron transport and CO₂ assimilation processes, chlorophyll bleaching and lipid peroxidation of leaves. Our results demonstrated that the t‐BuOOH‐selected DH plants exhibited enhanced tolerance not only to oxidative stress‐induced by t‐BuOOH but also to cold and drought stresses. In addition, they showed elevated activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase and glutathione S‐transferase when compared with the DH lines derived from microspores that were not exposed to t‐BuOOH and to the original hybrid plants. The results suggest that the simultaneous up‐regulation of several antioxidant enzymes may contribute to the oxidative and cold stress tolerance of the t‐BuOOH‐selected DH lines, and that the in vitro microspore selection represents a potential way to improve abiotic stress tolerance in maize.  相似文献   

Testing the ability of unmanned aerial systems and machine learning to map weeds at subfield scales: a test with the weed Alopecurus myosuroides (Huds)     

James PT Lambert  Dylan Z Childs  Rob P Freckleton 《Pest management science》2019,75(8):2283-2294

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Role of integrons in the proliferation of multiple drug resistance in selected bacteria occurring in poultry production     

P. Racewicz  M. Majewski  Z. E. Madeja  A. Łukomska  M. Kubiak 《British poultry science》2020,61(2):122-131

ABSTRACT

• 1. The increase in microbial resistance, and in particular multiple drug resistance (MDR), is an increasing threat to public health. The uncontrolled use of antibiotics and antibacterial chemotherapeutics in the poultry industry, especially in concentrations too low to cause inhibition, and the occurrence of residues in feed and in the environment play a significant role in the development of resistance among zoonotic food-borne microorganisms.

• 2. Determining the presence and transmission methods of resistance in bacteria is crucial for tracking and preventing antibiotic resistance. Horizontal transfer of genetic elements responsible for drug resistance is considered to be the main mechanism for the spread of antibiotic resistance.

• 3. Of the many well-known genetic elements responsible for horizontal gene transfer, integrons are among the most important factors contributing to multiple drug resistance. The mechanism of bacterial drug resistance acquisition through integrons is one of the essential elements of MDR prevention in animal production.

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Effects of formic acid and potassium diformate on the fermentation quality,chemical composition and aerobic stability of alfalfa silage          下载免费PDF全文

X. Yuan  A. Wen  Z. Dong  S. T. Desta  T. Shao 《Grass and Forage Science》2017,72(4):833-839

The objective of this study was to evaluate the efficacy of potassium diformate (KDF) as a potential additive for alfalfa silage. Fresh alfalfa was untreated or treated with formic acid (4 g/kg fresh weight, FW) or three concentrations of KDF (4, 5.5 or 7 g/kg FW). After 60 days of ensiling, the addition of formic acid and greater levels of KDF (5.5 and 7 g/kg) effectively reduced silage pH and inhibited the undesirable bacteria, indicated by lower butyric acid, ethanol, ammonia N concentrations and microbial populations (including enterobacteria, yeasts, moulds and clostridia). Additives decreased the dry‐matter loss, and more water‐soluble carbohydrates were preserved in the silages with formic acid or potassium diformate than in the control. Alfalfa silages treated with formic acid at 4 g/kg FW or potassium diformate at 5.5 or 7 g/kg FW were classified as the highest quality silage based on the higher Flieg's point (above 70) and remained stable for more than 9 days during aerobic exposure. Potassium diformate is recommended as an effective additive for alfalfa silages at a level of 5.5 or 7 g/kg FW under the humid and hot conditions of southern China.  相似文献   

Amplification of the <Emphasis Type="Italic">Phytophthora infestans</Emphasis> RG57 loci Facilitates <Emphasis Type="Italic">in planta</Emphasis> T-RFLP Identification of Late Blight Genotypes     

Champa P. Wijekoon  Binod B. Pageni  Melanie L. Kalischuk  Newton Z. Lupwayi  Lawrence M. Kawchuk 《American Journal of Potato Research》2017,94(3):251-257

Late blight, caused by the oomycete Phytophthora infestans (Mont.) de Bary, is a devastating disease in potato and tomato and causes yield and quality losses worldwide. The disease first emerged in central America and has since spread in North America including the United States and Canada. Several new genotypes of P. infestans have recently emerged, including US-22, US-23 and US-24. Due to significant economic and environmental impacts, there has been an increasing interest in the rapid identification of P. infestans genotypes. In addition to providing details regarding the various phenotypic characteristics such as fungicide resistance, host preference, and pathogenicity associated with various P. infestans genotypes, information related to pathogen movement and potential recombination may also be determined from the genetic analyses. Restriction fragment length polymorphism (RFLP) analysis with the RG57 loci is one of the most reliable procedures used to genotype P. infestans. However, the RFLP procedure requires propagation and isolation of the pathogen and relatively large amounts of DNA. Isolation of the late blight pathogen is sometimes impossible due to the poor condition of the infected tissues or the presence of fungicide residues. In this study, we describe a procedure to identify P. infestans at the molecular level in planta using terminal restriction fragment length polymorphism (T-RFLP) of the RG57 loci. This T-RFLP assay is sufficiently sensitive to detect and differentiate P. infestans genotypes directly in planta without propagation and isolation of the pathogen, to facilitate the timely implementation of best management practices.  相似文献   

Tritium Distribution in Soil in the Area of “Atomic” Lake near the Semipalatinsk Test Site     

Timonova  L. V.  Lyakhova  O. N.  Lukashenko  S. N.  Aidarkhanov  A. O.  Kabdyrakova  A. M.  Serzhanova  Z. B. 《Eurasian Soil Science》2020,53(3):355-361

Eurasian Soil Science - Data on concentrations and distribution of tritium in soils of the “Atomic” Lake excavation explosion area of Semipalatinsk Nuclear Test Site (Kazakhstan, East...  相似文献   

不同苦荞品种花和籽粒空间分布特征及差异分析     

马成瑞  向达兵  万燕  欧阳建勇  宋月  唐正松  刘建英  赵钢 《作物杂志》2020,36(1):35-45

以8个苦荞品种为试验材料,研究不同苦荞品种花和籽粒在植株不同部位的分布特征及差异。结果表明,不同苦荞品种花和籽粒总数存在显著差异,其中川荞2号总花数、总籽粒数最高,分别为3 046.6和1 367.8,而川荞1号最少,分别为1 017.6和487.2,品种间总花数和总籽粒数变异系数分别为34.8%、29.0%。不同苦荞品种花和籽粒空间分布呈现相同趋势,均主要分布于分枝上,分别占整株的76.3%和74.2%以上,且均显著高于主茎,分别高68.7%~87.0%和65.2%~84.5%。生产上可通过调控分枝的有效籽粒数来提高苦荞产量。  相似文献   

Evaluation of Assouline–Or Adjusted Model to Express Soil Drainage Curve     

Asadi  Z.  Mohammadi  M. H.  Shorafa  M.  Farhbakhsh  M.  Ghezelbash  E. 《Eurasian Soil Science》2020,53(6):749-759

Eurasian Soil Science - The objectives of this research are prediction of soil drainage curve using Assouline and Or (2014) adjusted soil drainage model and comparison of the predicted results with...  相似文献   

An updated review: Laboratory investigation of equine renal disease     

Z. Gratwick 《Equine Veterinary Education》2021,33(10):546-555

A variety of laboratory tests are available to assist the clinician in the assessment of kidney health and function. The majority of widely used tests are indicative of altered renal function, not becoming abnormal until function is significantly compromised. These include serum urea and creatinine concentrations, serum electrolyte concentrations, urine specific gravity (USG), fractional electrolyte excretion ratios and symmetric dimethylarginine (SDMA) concentrations. Some of these parameters may also be affected by nonrenal disease. The results of a further group of tests can indicate renal damage, but do not reflect renal function. These include urine tubular enzyme concentrations, urine protein concentrations, urine neutrophil gelatinase-associated lipocalin (NGAL) concentrations and cytological abnormalities.  相似文献   

Design and Experiment of Slave Computer Control System for Applying Variable-rate Liquid Fertilizer     

Zhang Ying-zi  Chen Hai-tao  Hou Shou-yin  Ji Wen-yi  Ouyang Bin-lin  Dun Guo-qiang  Zhang Ji-cheng 《东北农业大学学报(英文版)》2015,(2):73-79

In order to increase the applying rate of liquid fertilizer and reduce environmental pollution, a slave computer control system for applying variable-rate liquid fertilizer was designed. The system used SMC as core processor and electrically controlled pressure regulator as execution component. The characteristic equation of the system was obtained by using classical control theory. Results indicated that the characteristic equation met the requirements of routh-criterion, which indicated the working process of the system was stable. Performance of the slave computer was verified via bench tests. Results demonstrated that there was no significant influence on the response from interclass error. The fertilization error was less than 0.9, and the fertilization accuracy was larger than 97%. The liquid fertilizer emitted by the fertilizing devices had no significant difference in uniformity, which met the demands of the slave computer control system for applying variable-rate liquid fertilizer.  相似文献